rabbit anti hsp60  (Proteintech)

Journal: ACS Omega

Article Title: Cold Atmospheric Plasma Promotes Anti-Inflammatory and Regenerative Responses in Oral Soft Tissue through Redox-Driven Mitochondrial Regulation

doi: 10.1021/acsomega.5c11346

Figure Lengend Snippet: Cold atmospheric plasma (CAP) restores mitochondrial function in LPS-stimulated HGF-1 cells. (A) CAP maintained mitochondrial membrane potential, evaluated via TMRM staining, in inflammatory conditions. 200× magnification. The scale bar is 50 μm. (B) CAP reduced mitochondrial ROS (mtROS) levels, as evidenced by MitoSOX Red fluorescence. The original magnification was 200×, and the scale bar was 50 μm. (C) Quantification of ATP production showed that CAP treatment (30 s) markedly increased cellular ATP levels compared with the LPS-only group and even exceeded those of the untreated control. (D) Immunofluorescence staining of HSP60 showed that the shape and content of mitochondria improved after CAP exposure. (E) An analysis of mRNA expression showed that CAP increased the levels of PGC-1α, Nrf-1, and TFAM. (F) Western blot analysis confirmed the upregulated expression of PGC-1α, Nrf-1, and TFAM proteins in LPS-stimulated HGF-1 cells following CAP treatment. Values are shown as mean ± SD, and statistical significance was considered at * p < 0.05, ** p < 0.01, and *** p < 0.001 when compared with the control group.

Article Snippet: Primary antibodies against NF-κB p65 (8242S), phospho-NF-κB p65 (3033S), PGC-1α (2178S), TFAM (8076S), and HSP60 (12165) were from Cell Signaling Technology (Danvers, MA, USA), while β-actin (ab8224) and collagen type I (ab34710) were from Abcam (Cambridge, U.K.).

Techniques: Clinical Proteomics, Membrane, Staining, Fluorescence, Control, Immunofluorescence, Expressing, Western Blot

Journal: BMC Immunology

Article Title: SIRT5 affects lipid accumulation in cells of a metabolic-associated fatty liver disease model by regulating the succinylation status of HSPD1

doi: 10.1186/s12865-025-00776-7

Figure Lengend Snippet: SIRT5 leads to HSPD1 desuccinylation. A - B Western blot was carried out to evaluate the HSPD1 protein levels and the succinylation level of HSPD1. C - D co-IP was performed to verify the interaction between SIRT5 and HSPD1. E The co-locationof SIRT5 and HSPD1 obtained by the immunofluorescence method. F SPR analysis of the direct binding between SIRT5 and HSPD1. G - H Representative protein bands and quantitative analysis of protein degradation of HSPD1 when SIRT5 was overexpressed. *** p < 0.001

Article Snippet: Rabbit anti-KAT2A monoclonal antibody (#3305, 1:1000), rabbit anti-KAT3B monoclonal antibody (#4771, 1:1000), anti-CPT1A antibody (#97361, 1:1000), anti-SIRT5 antibody (#8779, 1:1000), anti-SIRT7 antibody (#5360, 1:1000), rabbit anti-HSPD1 monoclonal antibody (#12165, 1:1000), and anti-GAPDH antibody (#2118, 1:1000) were obtained from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Western Blot, Co-Immunoprecipitation Assay, Immunofluorescence, Binding Assay

Journal: BMC Immunology

Article Title: SIRT5 affects lipid accumulation in cells of a metabolic-associated fatty liver disease model by regulating the succinylation status of HSPD1

doi: 10.1186/s12865-025-00776-7

Figure Lengend Snippet: SIRT5-mediated hepatic steatosis function is reversed by the elevation of HSPD1. A PCR analysis was carried out to evaluate the mRNA levels of HSPD1 in HepG2 and Huh7 cells after transfection. *** p < 0.001. B - C TG and TC content of HepG2 and Huh7 cells of each group were accessed by ELISA. *** p < 0.001 (vs. control), # p < 0.05, ## p < 0.01, ### p < 0.001 (vs. FFA + oe-NC). D - G Images and quantitative analysis of Oil red O staining and BODIPY staining of HepG2 and Huh7 cells. *** p < 0.001 (vs. control), # p < 0.05, ### p < 0.001 (vs. FFA + oe-NC)

Article Snippet: Rabbit anti-KAT2A monoclonal antibody (#3305, 1:1000), rabbit anti-KAT3B monoclonal antibody (#4771, 1:1000), anti-CPT1A antibody (#97361, 1:1000), anti-SIRT5 antibody (#8779, 1:1000), anti-SIRT7 antibody (#5360, 1:1000), rabbit anti-HSPD1 monoclonal antibody (#12165, 1:1000), and anti-GAPDH antibody (#2118, 1:1000) were obtained from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Transfection, Enzyme-linked Immunosorbent Assay, Control, Staining